WSU-Pre-B-ALL, a new human EBV negative pre-B-Acute Lymphoblastic Leukemia, was established from frozen peripheral blood of a patient with ALL in leukemic phase.  Both the frozen cells and the established cell line exhibit t (1;19) chromosomal translocation, partial deletion of the short arm (p) of chromosome 9 and a loss of chromosome 13 as clonal abnormalities.